POU2AF1 antibody (C-Term)

Catalog No. ABIN374274

This anti-POU2AF1 antibody is a Goat Polyclonal antibody detecting POU2AF1 in WB. Suitable for Human, Rat, Mouse and Dog.

Quick Overview for POU2AF1 antibody (C-Term) (ABIN374274)

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This POU2AF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POU2AF1 Antibody

Binding Specificity: C-Term

Sequence: C-DAYALNHTLS VEGF

Specificity: This antibody detects POU2AF1 / BOB1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Dog, CowSpecies reactivity (tested):Human, Mouse

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006226.2 Genename: POU2AF1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 30 kDa band seen in mouse spleen lysate (Predicted MW of20 kDa according to NP_006226).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Alternative Name: POU2AF1 / BOB1

Background: Synonyms: B-cell-specific coactivator OBF-1, OBF-1, OBF1, OCA-B, OCAB, OCT-binding factor 1, POU domain class 2-associating factor 1

Gene ID: 5450

NCBI Accession: NP_006226

UniProt: Q16633